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Neuroreport. 2014 May 7;25(7):501-6. doi: 10.1097/WNR.0000000000000126.

Large-scale imaging of subcellular calcium dynamics of cortical neurons with G-CaMP6-actin.

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1
aGraduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo bBrain Science Institute, Saitama University, Saitama cCenter for Information and Neural Networks, Osaka, Japan.

Abstract

Understanding the information processing performed by a single neuron requires the monitoring of physiological dynamics from a variety of subcellular compartments including dendrites and axons. In this study, we showed that the expression of a fusion protein, consisting of a Ca²⁺ indicator protein (G-CaMP6) and a cytoskeleton protein (actin), enabled large-scale recording of Ca²⁺ dynamics from hundreds of postsynaptic spines and presynaptic boutons in a cortical pyramidal cell. At dendritic spines, G-CaMP6-actin had the potential to detect localized Ca²⁺ activity triggered by subthreshold synaptic inputs. Back-propagating action potentials reliably induced Ca²⁺ fluorescent increases in all spines. At axonal boutons, G-CaMP6-actin reported action potential trains propagating along axonal collaterals. The detectability of G-CaMP6-actin should contribute toward a deeper understanding of neural network architecture and dynamics at the level of individual synapses.

PMID:
24468806
DOI:
10.1097/WNR.0000000000000126
[Indexed for MEDLINE]
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