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Nat Rev Genet. 2014 Mar;15(3):205-13. doi: 10.1038/nrg3645. Epub 2014 Jan 28.

Ribosome profiling: new views of translation, from single codons to genome scale.

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Department of Embryology, Carnegie Institution, 3520 San Martin Drive, Baltimore, Maryland 21218, USA. Present address: Department of Molecular and Cell Biology, University of California, Berkeley, Barker Hall 422, Berkeley, California 94720, USA.


Genome-wide analyses of gene expression have so far focused on the abundance of mRNA species as measured either by microarray or, more recently, by RNA sequencing. However, neither approach provides information on protein synthesis, which is the true end point of gene expression. Ribosome profiling is an emerging technique that uses deep sequencing to monitor in vivo translation. Studies using ribosome profiling have already provided new insights into the identity and the amount of proteins that are produced by cells, as well as detailed views into the mechanism of protein synthesis itself.

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