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Exp Parasitol. 2014 Mar;138:25-9. doi: 10.1016/j.exppara.2014.01.005. Epub 2014 Jan 24.

A new approach for the characterization of proliferative cells in cestodes.

Author information

1
Sección Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
2
Sección Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany.
3
Unidad de Biología Celular, Institut Pasteur de Montevideo, Montevideo, Uruguay.
4
Sección Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; Departamento de Genética, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
5
Departamento de Genética, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
6
Sección Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. Electronic address: estelacasti@gmail.com.

Abstract

Cestodes show a remarkable proliferative capability that sustains the constant growth and differentiation of proglottids essential for their lifestyle. It is believed that a separate population of undifferentiated stem cells (the so-called germinative cells) are the only cells capable of proliferation during growth and development. The study of this particular cell subpopulation is hampered by the current lack of methods to isolate it. In this work, we developed a reproducible flow cytometry and cell sorting method to quantify and isolate the proliferating cells in the tetrathyridia larvae of the model cestode Mesocestoides corti, based on the DNA content of the cells. The isolated cells display the typical germinative cell morphology, and can be used for RNA isolation with a yield in the ng to μg range. We expect that this approach may facilitate the characterization of the germinative cells in M. corti and other model tapeworms.

KEYWORDS:

Cestodes; FACS; Flow cytometry; Mesocestoides corti; Proliferative cells

PMID:
24468551
DOI:
10.1016/j.exppara.2014.01.005
[Indexed for MEDLINE]
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