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J Vet Intern Med. 2014 Mar-Apr;28(2):571-5. doi: 10.1111/jvim.12277. Epub 2014 Jan 27.

Coagulation factor and hemostatic protein content of canine plasma after storage of whole blood at ambient temperature.

Author information

1
Pet Blood Bank UK, Loughborough Technology Centre, Loughborough, UK.

Abstract

BACKGROUND:

Standard practice in canine blood banking is to produce fresh frozen plasma (FFP) by separating and freezing plasma produced from blood within 8 hours of collection. Within canine blood donation programs, this can limit the number of units collected.

HYPOTHESIS/OBJECTIVES:

The aim was to compare the coagulation factor and hemostatic protein content (CF&HPC) of plasma produced from blood stored at ambient temperature for 8, 12, and 24 hours. Another aim was to compare the CF&HPC between Greyhound types and other breeds.

ANIMALS:

None.

METHODS:

In vitro study. A convenience sample of 58 units of canine blood from a blood donor pool was processed to prepare and freeze plasma 8, 12, or 24 hours following collection.

RESULTS:

Regardless of time of processing, the units contained therapeutic CF&HPC. Frozen plasma prepared after 24 hours had significantly higher factor VIII (P = .014) and factor X (P = .03) when compared with the frozen plasma prepared at 8 hours. Factor X (P < .01), fibrinogen (P < .01), and vWF (P = .04) were significantly lower in plasma collected from Greyhound types than in plasma collected from other breeds.

CONCLUSIONS AND CLINICAL IMPORTANCE:

Storing whole blood for up to 24 hours is a suitable method for producing FFP. Lower values for some coagulation factors and hemostatic proteins in plasma produced from Greyhound types would not preclude these dogs as FFP donors.

KEYWORDS:

Factor VIII; Hemostasis; Transfusion; von Willebrand factor

PMID:
24467263
PMCID:
PMC4858025
DOI:
10.1111/jvim.12277
[Indexed for MEDLINE]
Free PMC Article

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