Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2014 Jan 23;9(1):e86098. doi: 10.1371/journal.pone.0086098. eCollection 2014.

A novel method to identify routes of hepatitis C virus transmission.

Author information

1
Inserm 955, Hopital Henri Mondor, Créteil, France.
2
Institut Pasteur, Unité d'Epidémiologie des Maladies Emergentes, Paris, France.
3
Unité INSERM 669, La maison de Solenn, Paris, France.
4
Department of Community, Environmental and Occupational Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
5
National Liver Institute, Menoufia University, Shebeen El-Kom, Menufia, Egypt ; Viral Hepatitis Research Laboratory, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
6
Unité INSERM 550, Paris, France.
7
Viral Hepatitis Research Laboratory, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt ; Department of Microbiology, Faculty of Medicine, Minia University, Minya, Egypt.
8
Unité INSERM 785, Centre Hépato-Biliaire, Villejuif, France.
9
Institut Pasteur, Unité d'Epidémiologie des Maladies Emergentes, Paris, France ; Conservatoire National des Arts et Métiers, Chaire Santé et Développement, Paris, France.

Abstract

BACKGROUND:

We propose a new approach based on genetic distances among viral strains to infer about risk exposures and location of transmission at population level.

METHODS:

We re-analysed 133 viral sequences obtained during a cross-sectional survey of 4020 subjects living in a hepatitis C virus (HCV) endemic area in 2002. A permutation test was used to analyze the correlation between matrices of genetic distances in the NS5b region of all pairwise combinations of the 133 viral strains and exposure status (jointly exposed or not) to several potential HCV risk factors.

RESULTS:

Compared to subjects who did not share the same characteristics or iatrogenic exposures, the median Kimura genetic distances of viral strains were significantly smaller between brothers and sisters (0.031 versus 0.102, P<0.001), mother and child (0.044 versus 0.102, P<0.001), father and child (0.045 versus 0.102, P<0.001), or subjects exposed to periodontal treatment (0.084 versus 0.102, P = 0.02). Conversely, viral strains were more divergent between subjects exposed to blood transfusions (0.216 versus 0.102, P = 0.04) or tooth filling or extraction (0.108, versus 0.097, P = 0.05), suggesting acquisition of the virus outside of the village.

CONCLUSION:

This method provided insights on where infection took place (household, village) for several socio-demographic characteristics or iatrogenic procedures, information of great relevance for targeting prevention interventions. This method may have interesting applications for virologists and epidemiologists studying transmission networks in health-care facilities or among intravenous drug users.

PMID:
24465895
PMCID:
PMC3900465
DOI:
10.1371/journal.pone.0086098
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center