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Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2379-84. doi: 10.1073/pnas.1317360111. Epub 2014 Jan 24.

Natural variation of rice strigolactone biosynthesis is associated with the deletion of two MAX1 orthologs.

Author information

1
Laboratory of Plant Physiology, Wageningen University, 6708 PB, Wageningen, The Netherlands.

Erratum in

  • Proc Natl Acad Sci U S A. 2014 April 29;111(17):6528.

Abstract

Rice (Oryza sativa) cultivar Azucena--belonging to the Japonica subspecies--exudes high strigolactone (SL) levels and induces high germination of the root parasitic plant Striga hermonthica. Consistent with the fact that SLs also inhibit shoot branching, Azucena is a low-tillering variety. In contrast, Bala, an Indica cultivar, is a low-SL producer, stimulates less Striga germination, and is highly tillered. Using a Bala × Azucena F6 population, a major quantitative trait loci--qSLB1.1--for the exudation of SL, tillering, and induction of Striga germination was detected on chromosome 1. Sequence analysis of the corresponding locus revealed a rearrangement of a 51- to 59-kbp stretch between 28.9 and 29 Mbp in the Bala genome, resulting in the deletion of two cytochrome P450 genes--SLB1 and SLB2--with high homology to the Arabidopsis SL biosynthesis gene, MAX1. Both rice genes rescue the Arabidopsis max1-1 highly branched mutant phenotype and increase the production of the SL, ent-2'-epi-5-deoxystrigol, when overexpressed in Bala. Furthermore, analysis of this region in 367 cultivars of the publicly available Rice Diversity Panel population shows that the rearrangement at this locus is a recurrent natural trait associated with the Indica/Japonica divide in rice.

KEYWORDS:

CYP450; QTL; plant hormone

PMID:
24464483
PMCID:
PMC3926036
DOI:
10.1073/pnas.1317360111
[Indexed for MEDLINE]
Free PMC Article

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