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FEBS Lett. 2014 Mar 3;588(5):713-9. doi: 10.1016/j.febslet.2014.01.014. Epub 2014 Jan 23.

Effects of palmitoylation on the diffusional movement of BKCa channels in live cells.

Author information

1
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
2
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; National Leading Research Laboratory, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
3
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; National Leading Research Laboratory, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea; Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea. Electronic address: cspark@gist.ac.kr.

Abstract

BKCa channels are palmitoylated at a cluster of cysteine residues within the cytosolic linker connecting the 1st and 2nd transmembrane domains, and this lipid modification affects their surface expression. To verify the effects of palmitoylation on the diffusional dynamics of BKCa channels, we investigated their lateral movement. Compared to wild-type channels, the movement of mutant palmitoylation-deficient channels was much less confined and close to random. The diffusion of the mutant channel was also much faster than that of the wild type. Thus, the lateral movement of BKCa channels is greatly influenced by palmitoylation.

KEYWORDS:

BK(Ca) channel; Lateral diffusion; Lipid raft; Palmitoylation; Quantum dot; Single molecule

PMID:
24462688
DOI:
10.1016/j.febslet.2014.01.014
[Indexed for MEDLINE]
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