Format

Send to

Choose Destination
Prog Lipid Res. 2014 Apr;54:14-31. doi: 10.1016/j.plipres.2014.01.002. Epub 2014 Jan 24.

Application of stable isotopes to investigate the metabolism of fatty acids, glycerophospholipid and sphingolipid species.

Author information

1
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany.
2
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany. Electronic address: gerhard.liebisch@klinik.uni-regensburg.de.

Abstract

Nature provides an enormous diversity of lipid molecules that originate from various pathways. To gain insight into the metabolism and dynamics of lipid species, the application of stable isotope-labeled tracers combined with mass spectrometric analysis represents a perfect tool. This review provides an overview of strategies to track fatty acid, glycerophospholipid, and sphingolipid metabolism. In particular, the selection of stable isotope-labeled precursors and their mass spectrometric analysis is discussed. Furthermore, examples of metabolic studies that were performed in cell culture, animal and clinical experiments are presented.

KEYWORDS:

Lipid biosynthesis; Lipid metabolism; Lipidomics; Mass spectrometry; Metabolic profiling; Stable isotope labeling

PMID:
24462586
DOI:
10.1016/j.plipres.2014.01.002
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center