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Cell Rep. 2014 Feb 13;6(3):445-54. doi: 10.1016/j.celrep.2014.01.002. Epub 2014 Jan 23.

Recycling endosome tubule morphogenesis from sorting endosomes requires the kinesin motor KIF13A.

Author information

1
Institut Curie, Centre de Recherche, Paris 75248, France; Structure and Membrane Compartments, CNRS UMR144, Paris 75248, France. Electronic address: cedric.delevoye@curie.fr.
2
Institut Curie, Centre de Recherche, Paris 75248, France; Molecular Mechanisms of Intracellular Transport, CNRS UMR144, Paris 75248, France.
3
Institut Curie, Centre de Recherche, Paris 75248, France; Membrane and Cytoskeleton Dynamics, CNRS UMR144, Paris 75248, France.
4
Institut Curie, Centre de Recherche, Paris 75248, France; Structure and Membrane Compartments, CNRS UMR144, Paris 75248, France.
5
Institut Curie, Centre de Recherche, Paris 75248, France; Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS UMR144, Paris 75248, France.
6
Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia and University of Pennsylvania, Philadelphia, PA 19104, USA.
7
Institut Curie, Centre de Recherche, Paris 75248, France; Structure and Membrane Compartments, CNRS UMR144, Paris 75248, France; Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS UMR144, Paris 75248, France.

Abstract

Early endosomes consist of vacuolar sorting and tubular recycling domains that segregate components fated for degradation in lysosomes or reuse by recycling to the plasma membrane or Golgi. The tubular transport intermediates that constitute recycling endosomes function in cell polarity, migration, and cytokinesis. Endosomal tubulation and fission require both actin and intact microtubules, but although factors that stabilize recycling endosomal tubules have been identified, those required for tubule generation from vacuolar sorting endosomes (SEs) remain unknown. We show that the microtubule motor KIF13A associates with recycling endosome tubules and controls their morphogenesis. Interfering with KIF13A function impairs the formation of endosomal tubules from SEs with consequent defects in endosome homeostasis and cargo recycling. Moreover, KIF13A interacts and cooperates with RAB11 to generate endosomal tubules. Our data illustrate how a microtubule motor couples early endosome morphogenesis to its motility and function.

PMID:
24462287
PMCID:
PMC3928541
DOI:
10.1016/j.celrep.2014.01.002
[Indexed for MEDLINE]
Free PMC Article

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