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Cell. 2014 Jan 30;156(3):428-39. doi: 10.1016/j.cell.2013.12.015. Epub 2014 Jan 23.

Dividing cells regulate their lipid composition and localization.

Author information

1
Department of Biological Chemistry and Molecular Pharmacology and Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
2
Department of Chemistry and Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, UK.
3
Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, London WC2R 2LS, UK.
4
Department of Biological Chemistry and Molecular Pharmacology and Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA; Institute for Neurobiology, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany.
5
Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
6
Department of Biological Chemistry and Molecular Pharmacology and Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA; Department of Chemistry and Randall Division of Cell and Molecular Biophysics, King's College London, London SE1 1UL, UK. Electronic address: ulrike.eggert@kcl.ac.uk.

Abstract

Although massive membrane rearrangements occur during cell division, little is known about specific roles that lipids might play in this process. We report that the lipidome changes with the cell cycle. LC-MS-based lipid profiling shows that 11 lipids with specific chemical structures accumulate in dividing cells. Using AFM, we demonstrate differences in the mechanical properties of live dividing cells and their isolated lipids relative to nondividing cells. In parallel, systematic RNAi knockdown of lipid biosynthetic enzymes identified enzymes required for division, which highly correlated with lipids accumulated in dividing cells. We show that cells specifically regulate the localization of lipids to midbodies, membrane-based structures where cleavage occurs. We conclude that cells actively regulate and modulate their lipid composition and localization during division, with both signaling and structural roles likely. This work has broader implications for the active and sustained participation of lipids in basic biology.

PMID:
24462247
PMCID:
PMC3909459
DOI:
10.1016/j.cell.2013.12.015
[Indexed for MEDLINE]
Free PMC Article

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