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J Leukoc Biol. 1987 Nov;42(5):485-90.

Generation of macrophage variants with 5-azacytidine: selection for mannose receptor expression.

Author information

1
Department of Cell Biology and Physiology, Washington University Medical School, St. Louis, Missouri 63110.

Abstract

Mannose receptors are expressed only in primary macrophages. Established macrophage-derived cell lines, although apparently possessing the potential to synthesize mannose receptors, do not express them on their plasma membranes. Using the drug 5-Azacytidine, mannose receptor expression was induced in the macrophage-derived mouse cell line J774. Receptor positive cells were sorted through a fluorescent activated cell sorter (FACS) prior to cloning. Clones were isolated which continuously express mannose receptors in culture. These macrophages were able to endocytose beta-glucuronidase and phagocytose yeast particles via mannose receptors. Secretion of the lysosomal enzyme beta-hexosaminidase was also reduced in proportion to the degree of mannose receptor expression.

PMID:
2445884
DOI:
10.1002/jlb.42.5.485
[Indexed for MEDLINE]

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