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DNA Res. 2014 Jun;21(3):299-313. doi: 10.1093/dnares/dst058. Epub 2014 Jan 21.

The genome sequence of the highly acetic acid-tolerant Zygosaccharomyces bailii-derived interspecies hybrid strain ISA1307, isolated from a sparkling wine plant.

Author information

1
IBB-Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior Técnico, Department of Bioengineering, Universidade de Lisboa, Avenida Rovisco Pais, Lisbon 1049-001, Portugal.
2
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstrasse 1, Neuherberg D-85764, Germany.
3
Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga 4710-057, Portugal ICVS/3B's-PT Government Associate Laboratory, Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga 4710-057, Portugal.
4
Centre of Molecular and Environmental Biology (CBMA)/Department of Biology, University of Minho, Braga 4710-057, Portugal.
5
IBB-Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior Técnico, Department of Bioengineering, Universidade de Lisboa, Avenida Rovisco Pais, Lisbon 1049-001, Portugal isacorreia@tecnico.ulisboa.pt.

Abstract

In this work, it is described the sequencing and annotation of the genome of the yeast strain ISA1307, isolated from a sparkling wine continuous production plant. This strain, formerly considered of the Zygosaccharomyces bailii species, has been used to study Z. bailii physiology, in particular, its extreme tolerance to acetic acid stress at low pH. The analysis of the genome sequence described in this work indicates that strain ISA1307 is an interspecies hybrid between Z. bailii and a closely related species. The genome sequence of ISA1307 is distributed through 154 scaffolds and has a size of around 21.2 Mb, corresponding to 96% of the genome size estimated by flow cytometry. Annotation of ISA1307 genome includes 4385 duplicated genes (∼ 90% of the total number of predicted genes) and 1155 predicted single-copy genes. The functional categories including a higher number of genes are 'Metabolism and generation of energy', 'Protein folding, modification and targeting' and 'Biogenesis of cellular components'. The knowledge of the genome sequence of the ISA1307 strain is expected to contribute to accelerate systems-level understanding of stress resistance mechanisms in Z. bailii and to inspire and guide novel biotechnological applications of this yeast species/strain in fermentation processes, given its high resilience to acidic stress. The availability of the ISA1307 genome sequence also paves the way to a better understanding of the genetic mechanisms underlying the generation and selection of more robust hybrid yeast strains in the stressful environment of wine fermentations.

KEYWORDS:

Zygosaccharomyces bailii; genome sequencing and annotation; hybrid yeast strains; weak acid food preservatives tolerance; wine yeast strains

PMID:
24453040
PMCID:
PMC4060950
DOI:
10.1093/dnares/dst058
[Indexed for MEDLINE]
Free PMC Article

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