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Cryo Letters. 2013 Sep-Oct;34(5):508-19.

Development of cryopreservation for Loxocarya cinerea---an endemic Australian plant species important for post-mining restoration.

Author information

  • 1School of Biomedical Sciences, CHIRI Biosciences Research Precinct, Faculty of Health Sciences, Curtin University, GPO Box U1987, Perth WA 6845, Australia.
  • 2Botanic Gardens and Parks Authority, Fraser Avenue, West Perth, WA 6005, Australia.
  • 3Applied Physics, School of Applied Sciences, RMIT University, GPO Box 2476, Melbourne, VIC 3001, Australia.

Abstract

We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 +/- 5.9%; SXH804, 67.7 +/- 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 +/- 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (> 66.7% post LN survival) using the same protocol.

PMID:
24448771
[PubMed - indexed for MEDLINE]
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