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FEMS Yeast Res. 2014 May;14(3):399-411. doi: 10.1111/1567-1364.12138. Epub 2014 Feb 10.

Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae.

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Organization of Advanced Science and Technology, Kobe University, Kobe, Japan.


Yeast has the potential to be used in bulk-scale fermentative production of fuels and chemicals due to its tolerance for low pH and robustness for autolysis. However, expression of multiple external genes in one host yeast strain is considerably labor-intensive due to the lack of polycistronic transcription. To promote the metabolic engineering of yeast, we generated systematic and convenient genetic engineering tools to express multiple genes in Saccharomyces cerevisiae. We constructed a series of multi-copy and integration vector sets for concurrently expressing two or three genes in S. cerevisiae by embedding three classical promoters. The comparative expression capabilities of the constructed vectors were monitored with green fluorescent protein, and the concurrent expression of genes was monitored with three different fluorescent proteins. Our multiple gene expression tool will be helpful to the advanced construction of genetically engineered yeast strains in a variety of research fields other than metabolic engineering.


biofuel and biochemical production; genetic engineering; metabolic engineering; multiple gene expression

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