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J Med Primatol. 2014 Apr;43(2):59-71. doi: 10.1111/jmp.12097. Epub 2014 Jan 22.

Plasma antibody profiles in non-human primate tuberculosis.

Author information

1
Center for Comparative Medicine, University of California, Davis, CA, USA.

Abstract

BACKGROUND:

Tuberculosis (TB) in non-human primates (NHPs) is highly contagious, requiring efficient identification of animals infected with Mycobacterium tuberculosis. Tuberculin skin test is usually used but lacks desirable sensitivity/specificity and efficiency.

METHODS:

We aimed to develop an immunoassay for plasma antibodies against M. tuberculosis. A key challenge is that not all infected animals contain antibodies against the same M. tuberculosis antigen. Therefore, a multiplex panel of 28 antigens (Luminex(®) -Platform) was developed.

RESULTS:

Data revealed antibodies against eight antigens (Rv3875, Rv3875-Rv3874 fusion, Rv3874, Rv0934, Rv3881, Rv1886c, Rv2031, Rv3841) in experimentally infected (M. tuberculosis strains: Erdman and H37Rv) NHPs (rhesus and cynomolgus macaques). In a naturally acquired M. tuberculosis infection, rhesus macaques (n = 15) with lung TB pathology (n = 10) contained antibodies to five additional antigens (Rv0831, Rv2220, Rv0054, Rv1099, and Rv0129c).

CONCLUSIONS:

Results suggest that this user-friendly and easily implementable multiplex panel, containing 13 M. tuberculosis antigens, may provide a high-throughput alternative for NHP TB screening.

KEYWORDS:

antibodies; multiplex; tuberculosis

PMID:
24446897
DOI:
10.1111/jmp.12097
[Indexed for MEDLINE]

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