Format

Send to

Choose Destination
See comment in PubMed Commons below
J Nutr Biochem. 2014 Feb;25(2):201-7. doi: 10.1016/j.jnutbio.2013.10.007. Epub 2013 Nov 14.

Sulforaphane attenuates obesity by inhibiting adipogenesis and activating the AMPK pathway in obese mice.

Author information

1
College of Pharmacy and Center for Innovative Cancer Therapeutics, Chungbuk National University, 52 Naesudong-ro, Heungduk-gu, Cheongju 361-763, Republic of Korea.
2
Department of Medicine, Chungbuk National University, Cheongju, Republic of Korea.
3
Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
4
College of Pharmacy and Center for Innovative Cancer Therapeutics, Chungbuk National University, 52 Naesudong-ro, Heungduk-gu, Cheongju 361-763, Republic of Korea. Electronic address: yoohs@chungbuk.ac.kr.

Abstract

Obesity is associated with metabolic disorders. Sulforaphane, an isothiocyanate, inhibits adipogenesis and the occurrence of cardiovascular disease. In this study, we investigated whether sulforaphane could prevent high-fat diet (HFD)-induced obesity in C57BL/6N mice. Mice were fed a normal diet (ND), HFD or HFD plus 0.1% sulforaphane (SFN) for 6 weeks. Food efficiency ratios and body weight were lower in HFD-SFN-fed mice than in HFD-fed mice. SFN attenuated HFD-induced visceral adiposity, adipocyte hypertrophy and fat accumulation in the liver. Serum total cholesterol and leptin, and liver triglyceride levels were lower in HFD-SFN-fed mice than in HFD-fed mice. SFN decreased the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα) and leptin in the adipose tissue of HFD-SFN mice and increased adiponectin expression. Phosphorylation of AMP-activated protein kinase α (AMPKα) and acetyl-CoA carboxylase in the adipose tissue of HFD-SFN-fed mice was elevated, and HMG-CoA reductase expression was decreased compared with HFD-fed mice. Thus, these results suggest that SFN may induce antiobesity activity by inhibiting adipogenesis through down-regulation of PPARγ and C/EBPα and by suppressing lipogenesis through activation of the AMPK pathway.

KEYWORDS:

AMPK; Adipogenesis; Leptin; Lipogenesis; Obesity; Sulforaphane

PMID:
24445045
DOI:
10.1016/j.jnutbio.2013.10.007
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center