Format

Send to

Choose Destination
Curr Top Med Chem. 2014 Mar;14(5):624-9.

Cell adhesion response on femtosecond laser initiated liquid assisted silicon surface.

Author information

1
National Institute for Laser, Plasma and Radiation Physics, Laser Department, Atomistilor 409, P. O. Box MG-36, 077125 Magurele, Romania. magda.ulmeanu@inflpr.ro.

Abstract

Silicon substrates were irradiated at normal incidence with a femtosecond Ti:sapphire laser (Quatronix, 90 fs pulse duration, 1 kHz repetition rate, M(2) ~ 1.2, maximum energy peak 350 mJ ) operating at a wavelength of 400 nm and focused via a microscope objective (Newport; UV Objective Model, 37x 0.11 N.A.). The laser scanning was assisted by liquids precursors media such as methanol and 1,1,2-trichlorotrifluoroethane. By altering the processing parameters, such as incident laser energy, scanning speed, and different irradiation media, various surface structures were produced on areas with 1 mm(2) dimensions. We analyzed the dependence of the surface morphology on laser pulse energy, scanning speed and irradiation media. Well ordered areas are developed without imposing any boundary conditions for the capillary waves that coarsens the ripple pattern. To assess biomaterial-driven cell adhesion response we investigated actin filaments organization and cell morphological changes following growth onto processed silicon substrates. Our study of bone cell progenitor interaction with laser nanoprocessed silicon lines has shown that cells anchor mainly to contact points along the nanostructured surface. Consequently, actin filaments are stretched towards the 15 ┬Ám wide parallel lines increasing lateral cell spreading and changing the bipolar shape of mesenchymal stem cells.

PMID:
24444164
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Bentham Science Publishers Ltd.
Loading ...
Support Center