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Nat Methods. 2014 Mar;11(3):297-300. doi: 10.1038/nmeth.2809. Epub 2014 Jan 19.

Continuous throughput and long-term observation of single-molecule FRET without immobilization.

Author information

1
1] Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. [2].
2
1] Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. [2] [3].
3
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.

Abstract

We present an automated microfluidic platform that performs multisecond observation of single molecules with millisecond time resolution while bypassing the need for immobilization procedures. With this system, we confine biomolecules to a thin excitation field by reversibly collapsing microchannels to nanochannels. We demonstrate the power of our method by studying a variety of complex nucleic acid and protein systems, including DNA Holliday junctions, nucleosomes and human transglutaminase 2.

PMID:
24441935
DOI:
10.1038/nmeth.2809
[Indexed for MEDLINE]

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