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Indian J Pathol Microbiol. 2013 Oct-Dec;56(4):359-64. doi: 10.4103/0377-4929.125290.

Association of Epstein Barr virus deoxyribonucleic acid with lung carcinoma.

Author information

1
Department of Pathology, Quaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

CONTEXT:

Lung cancer is the leading cause of cancer death worldwide. In addition to smoking, a variety of other contributing factors, including viral infection, have been suggested in tumorigenesis. Epstein Barr virus (EBV), which is linked to various malignancies, seems to be a good candidate.

AIMS:

The aim of this study was to investigate the association of EBV with lung carcinomas.

SETTINGS AND DESIGN:

A total number of 90 formalin fixed paraffin embedded lung tissue samples including 48 cases of lung cancers (18 squamous cell carcinomas [SCCs], 18 adenocarcinomas and 12 small cell carcinomas) and 42 non-tumoral samples (control group), were retrieved from the pathology archive.

MATERIALS AND METHODS:

Following deoxyribonucleic acid extraction, polymerase chain reaction (PCR) was performed using an EBV-Eph PCR kit. The positive cases were studied immunohistochemically for the expression of EBV-late membrane protein-1 (EBV-LMP-1) in tumoral tissues.

STATISTICAL ANALYSIS USED:

The t-test and Fisher exact test were used and P < 0.05 was considered statistically significant.

RESULTS:

Five of our cases, including four SCCs and one adenocarcinoma and two control samples showed a positive reaction in PCR. All positive tumoral cases showed diffuse staining with LMP-1 in immunohistochemistry.

CONCLUSIONS:

We found a significant difference in the presence of the EBV genome in cases of lung SCC compared to other lung lesions (P = 0.02). According to our data, EBV is not at major play in the non-lymphoepithelioma-like cancers of the lung in general, but may have a role in the tumorigenesis of some lung SCCs.

PMID:
24441222
DOI:
10.4103/0377-4929.125290
[Indexed for MEDLINE]
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