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Curr Opin Neurobiol. 2014 Jun;26:88-95. doi: 10.1016/j.conb.2013.12.013. Epub 2014 Jan 14.

In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes.

Author information

1
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States.
2
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States; Department of Psychiatry, New York University Medical Center, New York, NY 10016, United States.
3
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States. Electronic address: gyorgy.buzsaki@nyumc.org.

Abstract

Identification and manipulation of different GABAergic interneuron classes in the behaving animal are important to understand their role in circuit dynamics and behavior. The combination of optogenetics and large-scale neuronal recordings allows specific interneuron populations to be identified and perturbed for circuit analysis in intact animals. A crucial aspect of this approach is coupling electrophysiological recording with spatially and temporally precise light delivery. Focal multisite illumination of neuronal activators and silencers in predetermined temporal configurations or a closed loop manner opens the door to addressing many novel questions. Recent progress demonstrates the utility and power of this novel technique for interneuron research.

PMID:
24440414
PMCID:
PMC4024355
DOI:
10.1016/j.conb.2013.12.013
[Indexed for MEDLINE]
Free PMC Article

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