Format

Send to

Choose Destination
Gene. 2014 Mar 15;538(1):46-55. doi: 10.1016/j.gene.2014.01.024. Epub 2014 Jan 14.

A novel MYB transcription factor, GmMYBJ1, from soybean confers drought and cold tolerance in Arabidopsis thaliana.

Author information

1
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China.
2
College of Life Science and Agroforestry, Qiqihaer University, Qiqihaer 161006, Heilongjiang, China.
3
Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130012, Jilin, China.
4
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China. Electronic address: wangying2009@jlu.edu.cn.
5
Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun 130062, Jilin, China. Electronic address: qywang@jlu.edu.cn.

Abstract

MYB transcription factors play important roles in the regulation of plant growth, developmental metabolism and stress responses. In this study, a new MYB transcription factor gene, GmMYBJ1, was isolated from soybean [Glycine max (L.)]. The GmMYBJ1 cDNA is 1296bp in length with an open reading frame (ORF) of 816 bp encoding for 271 amino acids. The amino acid sequence displays similarities to the typical R2R3 MYB proteins reported in other plants. Transient expression analysis using the GmMYBJ1-GFP fusion gene in onion epidermal cells revealed that the GmMYBJ1 protein is targeted to the nucleus. Quantitative RT-PCR analysis demonstrated that GmMYBJ1 expression was induced by abiotic stresses, such as drought, cold, salt and exogenous abscisic acid (ABA). Compared to wild-type (WT) plants, transgenic Arabidopsis overexpressing GmMYBJ1 exhibited an enhanced tolerance to drought and cold stresses. These results indicate that GmMYBJ1 has the potential to be utilized in transgenic breeding lines to improve abiotic stress tolerance.

KEYWORDS:

MYB transcription factor; Soybean; Stress tolerance; Transgenic Arabidopsis

PMID:
24440241
DOI:
10.1016/j.gene.2014.01.024
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center