Expression and clinical significance of tyrosine phosphatase SHP-2 in colon cancer

Biomed Pharmacother. 2014 Apr;68(3):285-90. doi: 10.1016/j.biopha.2013.10.012. Epub 2013 Nov 12.

Abstract

Protein-tyrosine phosphatase SHP-2, encoded by gene PTPN11, has been identified as a tumor-promoting factor in several types of leukemia and is hyper-activated by other mechanisms in some solid tumors including gastric cancer, breast cancer, non-small cell lung cancer (NSCLC), etc. But few were reported on the expression and significances of SHP-2 in colon cancer. Here, we detect SHP-2 expression in colon cancer cells, colon cancer-induced by AOM+DSS in mice and 232 human colon cancer specimens, including 58 groups of self-matched adjacent peritumor tissues and normal tissues. We found that compared to the normal colon tissues, SHP-2 significantly decreased in tumor tissues (P<0.001). The same results were got in colon tumor cells as well as mice colon tumors. And in humans samples, low SHP-2 expression showed a significantly correlation with poor tumor differentiation (P<0.05), late TNM stage (P=0.1666) and lymph node metastasis (P<0.05).

Keywords: Colon cancer; Expression; SHP-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Blotting, Western
  • Cell Line, Tumor
  • Colon / enzymology
  • Colonic Neoplasms / enzymology*
  • Colonic Neoplasms / pathology
  • Female
  • Humans
  • Immunohistochemistry
  • Lymphatic Metastasis
  • Male
  • Mice, Inbred C57BL
  • Middle Aged
  • Neoplasm Grading
  • Neoplasm Staging
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11 / biosynthesis
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11 / genetics*
  • Real-Time Polymerase Chain Reaction

Substances

  • PTPN11 protein, human
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11
  • Ptpn11 protein, mouse