Format

Send to

Choose Destination
Nat Rev Mol Cell Biol. 2014 Feb;15(2):95-107. doi: 10.1038/nrm3738. Epub 2014 Jan 17.

Synthetic biology in mammalian cells: next generation research tools and therapeutics.

Author information

1
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
2
1] Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA. [2] Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.

Abstract

Recent progress in DNA manipulation and gene circuit engineering has greatly improved our ability to programme and probe mammalian cell behaviour. These advances have led to a new generation of synthetic biology research tools and potential therapeutic applications. Programmable DNA-binding domains and RNA regulators are leading to unprecedented control of gene expression and elucidation of gene function. Rebuilding complex biological circuits such as T cell receptor signalling in isolation from their natural context has deepened our understanding of network motifs and signalling pathways. Synthetic biology is also leading to innovative therapeutic interventions based on cell-based therapies, protein drugs, vaccines and gene therapies.

PMID:
24434884
PMCID:
PMC4032074
DOI:
10.1038/nrm3738
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center