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Fungal Biol. 2014 Jan;118(1):32-47. doi: 10.1016/j.funbio.2013.10.011. Epub 2013 Nov 11.

Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis.

Author information

1
CIRAD, UMR AGAP, F-63000 Clermont-Ferrand, France; Clermont-Université, Université Blaise Pascal, UMR 547 PIAF, BP 10448, 63000 Clermont-Ferrand, France.
2
Clermont-Université, Université Blaise Pascal, UMR 547 PIAF, BP 10448, 63000 Clermont-Ferrand, France.
3
CIRAD, UMR AGAP, F-34398 Montpellier, France; CIRAD, UMR BGPI, F-34398 Montpellier, France.
4
CIRAD, UMR BGPI, F-34398 Montpellier, France.
5
Departamento de Agronomia, Universidade Estadual de Maringá, Maringá, PR CEP 8702-900, Brazil.
6
Biotechnology Unit, RRIM, Malaysian Rubber Board, P.O. Box 10150, 50908 Kuala Lumpur, Malaysia.
7
CIRAD, UMR AGAP, F-34398 Montpellier, France.
8
CIRAD, UMR AGAP, F-63000 Clermont-Ferrand, France; Clermont-Université, Université Blaise Pascal, UMR 547 PIAF, BP 10448, 63000 Clermont-Ferrand, France. Electronic address: valerie.pujade-renaud@cirad.fr.

Abstract

Corynespora cassiicola is an important plant pathogenic Ascomycete causing the damaging Corynespora Leaf Fall (CLF) disease in rubber tree (Hevea brasiliensis). A small secreted glycoprotein named cassiicolin was previously described as an important effector of C. cassiicola. In this study, the diversity of the cassiicolin-encoding gene was analysed in C. cassiicola isolates sampled from various hosts and geographical origins. A cassiicolin gene was detected in 47 % of the isolates, encoding up to six distinct protein isoforms. In three isolates, two gene variants encoding cassiicolin isoforms Cas2 and Cas6 were found in the same isolate. A phylogenetic tree based on four combined loci and elucidating the diversity of the whole collection was strongly structured by the toxin class, as defined by the cassiicolin isoform. The isolates carrying the Cas1 gene (toxin class Cas1), all grouped in the same highly supported clade, were found the most aggressive on two rubber tree cultivars. Some isolates in which no Cas gene was detected could nevertheless generate moderate symptoms, suggesting the existence of other yet uncharacterized effectors. This study provides a useful base for future studies of C. cassiicola population biology and epidemiological surveys in various host plants.

KEYWORDS:

Corynespora Leaf Fall; Pathogen effector; Phylogeny; Rubber tree; Toxin class

PMID:
24433675
DOI:
10.1016/j.funbio.2013.10.011
[Indexed for MEDLINE]

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