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Diagn Microbiol Infect Dis. 2014 Mar;78(3):292-4. doi: 10.1016/j.diagmicrobio.2013.12.003. Epub 2013 Dec 14.

Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in Turkey.

Author information

1
Department of Medical Microbiology, Faculty of Medicine, Bezmialem Vakıf University, Istanbul, Turkey.
2
Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Gümüşhane University, 29100 Gumushane, Turkey.
3
Department of Medical Microbiology, Recep Tayyip Erdogan University Faculty of Medicine, Rize, Turkey.
4
Service de Bactériologie-Virologie, INSERM U914, Emerging resistance to antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K-Bicêtre, France.
5
Department of Biology, Faculty of Arts & Sciences, Artvin Coruh University, 08000 Artvin, Turkey.
6
Department of Biology, Faculty of Arts & Sciences, Recep Tayyip Erdogan University, Rize, Turkey. Electronic address: cemal.sandalli@erdogan.edu.tr.

Abstract

Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = 1). PCR and DNA sequence analysis revealed that 1 strain possessed the blaGES-5 and another carried a novel blaVIM variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey.

KEYWORDS:

Carbapenemase; Class 1 integron; ESBL; Pseudomonas aeruginosa; β-lactamase

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