Organic selenium supplementation increases PHGPx but does not improve viability in chilled boar semen

Andrologia. 2015 Feb;47(1):85-90. doi: 10.1111/and.12226. Epub 2014 Jan 16.

Abstract

This study evaluated the effects of dietary organic selenium (Se) on viability of chilled boar semen. Twelve boars were divided into three groups: control (CON), 0.3 mg kg(-1) sodium selenite; inorganic (INO), 0.5 mg kg(-1) sodium selenite and organic (ORG), 0.5 mg kg(-1) Se yeast. The experiment was conducted within 10 weeks, and analysis was performed fortnightly, in storage semen by 72 h. No effect was observed on motility; however, straightness and linearity percentages were higher (P < 0.05) in the animals receiving CON diet compared with INO group. Percentages of cells with both plasma and acrosomal intact membranes, lipidic membrane peroxidation and mitochondrial membrane potential were similar on all treatments. Animals receiving CON diet presented higher (P < 0.05) values of ATP when compared with INO group. The PHGPx was higher (P < 0.05) in animals that received ORG in comparison with INO group. In conclusion, organic selenium supplementation increases PHGPx but does not improve chilled semen viability in 72 h.

Keywords: Chilled boar semen; PHGPx; organic selenium; sperm motility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrosome / drug effects
  • Animals
  • Antioxidants / pharmacology*
  • Cell Survival / drug effects
  • Dietary Supplements*
  • Glutathione Peroxidase / drug effects*
  • Glutathione Peroxidase / metabolism
  • Male
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Selenium / pharmacology*
  • Semen Analysis
  • Semen Preservation
  • Sperm Motility / drug effects
  • Spermatozoa / drug effects*
  • Spermatozoa / enzymology
  • Swine

Substances

  • Antioxidants
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Glutathione Peroxidase
  • Selenium