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Pest Manag Sci. 2014 Dec;70(12):1831-9. doi: 10.1002/ps.3725. Epub 2014 Mar 6.

Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean.

Author information

1
DuPont Pioneer, Hayward, CA, USA.

Abstract

BACKGROUND:

Sulfonylurea (SU) herbicides are effective because they inhibit acetolactate synthase (ALS), a key enzyme in branched-chain amino acid synthesis required for plant growth. A soybean line known as W4-4 was developed through rounds of seed mutagenesis and was demonstrated to have a high degree of ALS-based resistance to both post-emergence and pre-emergence applications of a variety of SU herbicides. This report describes the molecular and phenotypic characterization of the Als1 and Als2 mutations that confer herbicide resistance to SUs and other ALS inhibitors.

RESULTS:

The mutations are shown to occur in two different ALS genes that reside on different chromosomes: Als1 (P178S) on chromosome 4 and Als2 (W560L) on chromosome 6 (P197S and W574L in Arabidopsis thaliana).

CONCLUSION:

Although the Als1 and Als2 genes are unlinked, the combination of these two mutations is synergistic for improved tolerance of soybeans to ALS-inhibiting herbicides.

KEYWORDS:

acetohydroxyacid synthase; acetolactate synthase; herbicide resistance; herbicide tolerance; mutation; soybean; sulfonylurea

PMID:
24425499
PMCID:
PMC4282486
DOI:
10.1002/ps.3725
[Indexed for MEDLINE]
Free PMC Article
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