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Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):49-52. doi: 10.1107/S2053230X13031361. Epub 2013 Dec 24.

Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens.

Author information

1
Department of Chemistry, University of Eastern Finland, PO Box 111, 80101 Joensuu, Finland.
2
VTT Technical Research Centre of Finland, PO Box 1000, 02044 VTT, Finland.

Abstract

D-galacturonic acid is the main component of pectin. It could be used to produce affordable renewable fuels, chemicals and materials through biotechnical conversion. Keto-deoxy-D-galactarate (KDG) dehydratase is an enzyme in the oxidative pathway of D-galacturonic acid in Agrobacterium tumefaciens (At). It converts 3-deoxy-2-keto-L-threo-hexarate to α-ketoglutaric semialdehyde. At KDG dehydratase was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 169.1, b = 117.8, c = 74.3 Å, β = 112.4° and an asymmetric unit of four monomers. X-ray diffraction data were collected to 1.9 Å resolution using synchrotron radiation. The three-dimensional structure of At KDG dehydratase will provide valuable information on the function of the enzyme and will allow it to be engineered for biorefinery-based applications.

KEYWORDS:

Agrobacterium tumefaciens; d-galacturonic acid; keto-deoxy-d-galactarate dehydratase; oxidative pathway

PMID:
24419616
PMCID:
PMC3943101
DOI:
10.1107/S2053230X13031361
[Indexed for MEDLINE]
Free PMC Article

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