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Virology. 2014 Jan 20;449:96-103. doi: 10.1016/j.virol.2013.11.003. Epub 2013 Nov 27.

Inhibition of HIV by Legalon-SIL is independent of its effect on cellular metabolism.

Author information

1
Department of Laboratory Medicine, University of Washington, Seattle, WA, United States.
2
Department of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA, United States.
3
Department of Medicine (Division of Metabolism, Endocrinology, and Nutrition), University of Washington, Seattle, WA, United States.
4
Department of Laboratory Medicine, University of Washington, Seattle, WA, United States; Department of Global Health, University of Washington, Seattle, WA, United States. Electronic address: polyak@uw.edu.

Abstract

In this report, we further characterized the effects of silibinin (SbN), derived from milk thistle extract, and Legalon-SIL (SIL), a water-soluble derivative of SbN, on T cell metabolism and HIV infection. We assessed the effects of SbN and SIL on peripheral blood mononuclear cells (PBMC) and CEM-T4 cells in terms of cellular growth, ATP content, metabolism, and HIV infection. SIL and SbN caused a rapid and reversible (upon removal) decrease in cellular ATP levels, which was associated with suppression of mitochondrial respiration and glycolysis. SbN, but not SIL inhibited glucose uptake. Exposure of T cells to SIL (but not SbN or metabolic inhibitors) during virus adsorption blocked HIV infection. Thus, both SbN and SIL rapidly perturb T cell metabolism in vitro, which may account for its anti-inflammatory and anti-proliferative effects that arise with prolonged exposure of cells. However, the metabolic effects are not involved in SIL's unique ability to block HIV entry.

KEYWORDS:

HIV; Metabolism; Silibinin; Silymarin; T cell

PMID:
24418542
PMCID:
PMC3909448
DOI:
10.1016/j.virol.2013.11.003
[Indexed for MEDLINE]
Free PMC Article

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