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PLoS One. 2014 Jan 8;9(1):e84891. doi: 10.1371/journal.pone.0084891. eCollection 2014.

The non-JAZ TIFY protein TIFY8 from Arabidopsis thaliana is a transcriptional repressor.

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Department of Plant Systems Biology, VIB, Gent, Belgium ; Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium.
Plant-Microbe Interactions, Institute of Environmental Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
Department of Medical Protein Research, VIB, Gent, Belgium ; Department of Biochemistry, Ghent University, Gent, Belgium.


Jasmonate (JA) signalling is mediated by the JASMONATE-ZIM DOMAIN (JAZ) repressor proteins, which are degraded upon JA perception to release downstream responses. The ZIM protein domain is characteristic of the larger TIFY protein family. It is currently unknown if the atypical member TIFY8 is involved in JA signalling. Here we show that the TIFY8 ZIM domain is functional and mediated interaction with PEAPOD proteins and NINJA. TIFY8 interacted with TOPLESS through NINJA and accordingly acted as a transcriptional repressor. TIFY8 expression was inversely correlated with JAZ expression during development and after infection with Pseudomonas syringae. Nevertheless, transgenic lines with altered TIFY8 expression did not show changes in JA sensitivity. Despite the functional ZIM domain, no interaction with JAZ proteins could be found. In contrast, TIFY8 was found in protein complexes involved in regulation of dephosphorylation, deubiquitination and O-linked N-acetylglucosamine modification suggesting an important role in nuclear signal transduction.

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