Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2014 Jan 8;9(1):e84469. doi: 10.1371/journal.pone.0084469. eCollection 2014.

Comparative genomics of cultured and uncultured strains suggests genes essential for free-living growth of Liberibacter.

Author information

  • 1Microbiology and Cell Science Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida, United States of America.
  • 2Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, Illinois, United States of America.
  • 3Plant Pathology Department, Citrus Research and Development Center, Institute of Food and Agricultural Sciences, University of Florida, Lake Alfred, Florida, United States of America.

Abstract

The full genomes of two uncultured plant pathogenic Liberibacter, Ca. Liberibacter asiaticus and Ca. Liberibacter solanacearum, are publicly available. Recently, the larger genome of a closely related cultured strain, Liberibacter crescens BT-1, was described. To gain insights into our current inability to culture most Liberibacter, a comparative genomics analysis was done based on the RAST, KEGG, and manual annotations of these three organisms. In addition, pathogenicity genes were examined in all three bacteria. Key deficiencies were identified in Ca. L. asiaticus and Ca. L. solanacearum that might suggest why these organisms have not yet been cultured. Over 100 genes involved in amino acid and vitamin synthesis were annotated exclusively in L. crescens BT-1. However, none of these deficiencies are limiting in the rich media used to date. Other genes exclusive to L. crescens BT-1 include those involved in cell division, the stringent response regulatory pathway, and multiple two component regulatory systems. These results indicate that L. crescens is capable of growth under a much wider range of conditions than the uncultured Liberibacter strains. No outstanding differences were noted in pathogenicity-associated systems, suggesting that L. crescens BT-1 may be a plant pathogen on an as yet unidentified host.

PMID:
24416233
PMCID:
PMC3885570
DOI:
10.1371/journal.pone.0084469
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center