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J Craniomaxillofac Surg. 2014 Sep;42(6):825-34. doi: 10.1016/j.jcms.2013.11.020. Epub 2013 Dec 4.

Effects of different scaffolds on rat adipose tissue derived stroma cells.

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  • 1Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany.
  • 2Department of Oral & Maxillofacial Surgery, Christian-Albrechts-University, Arnold-Heller-Str. 16, 24105 Kiel, Germany. Electronic address: gierloff@mkg.uni-kiel.de.

Abstract

BACKGROUND:

Adipose tissue derived stroma cells (ASC's) offer for many advantages for tissue engineering strategies over mesenchymal stroma cells from other sources and ideal carrier materials have to be identified for them. The aim of this study was to demonstrate and to compare the effects of three clinically established biomaterials on proliferation and metabolic activity of rat ASC's in vitro.

MATERIALS AND METHODS:

Rat adipose tissue derived stroma cells (ASC's) were isolated and differentiated into distinct lineages proved by lineage specific staining and gene expression analysis (RT-PCR). The biomaterials Bio-Gide(®), Tutodent(®) Membrane and Belotero(®) Soft were tested with rat ASC's for their biocompatibility using scanning electron microscopy (SEM), cell vitality staining, cytotoxicity and proliferation tests (LDH, MTT, BrdU, WST-1).

RESULTS:

The collagen membrane Bio-Gide(®) resulted in a significantly higher viability and proliferation (WST-1, BrdU) compared to Tutodent(®) Membrane. No significant difference was determined in the LDH and MTT test. The hyaluronic acid gel Belotero(®) Soft showed no cytotoxicity (LDH, FDA/PI) and had no negative effects on metabolic activity (WST-1, MTT) or cell proliferation (BrdU) of ASC's.

CONCLUSION:

Our results indicate Bio-Gide(®) and Belotero(®) Soft as preferable carrier materials for ASC's. For the further establishment of ASC's-based treatment strategies, in vivo investigations on the tissue regeneration potential of these cell-biomaterial scaffolds should follow.

Copyright © 2013 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

KEYWORDS:

Adipose tissue derived stroma cells (ASC's); Biocompatibility; Collagen membranes; Hyaluronic acid gel; Multilineage potential; Scaffold; Tissue engineering

[PubMed - indexed for MEDLINE]
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