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J Biomol NMR. 2014 Feb;58(2):83-99. doi: 10.1007/s10858-013-9807-x. Epub 2014 Jan 10.

SARA: a software environment for the analysis of relaxation data acquired with accordion spectroscopy.

Author information

1
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 701 Hunterian, 725 North Wolfe St., Baltimore, MD, 21205, USA.

Abstract

We present SARA (Software for Accordion Relaxation Analysis), an interactive and user-friendly MATLAB software environment designed for analyzing relaxation data obtained with accordion spectroscopy. Accordion spectroscopy can be used to measure nuclear magnetic resonance (NMR) relaxation rates in a fraction of the time required by traditional methods, yet data analysis can be intimidating and no unified software packages are available to assist investigators. Hence, the technique has not achieved widespread use within the NMR community. SARA offers users a selection of analysis protocols spanning those presented in the literature thus far, with modifications permitting a more general application to crowded spectra such as those of proteins. We discuss the advantages and limitations of each fitting method and suggest a protocol combining the strengths of each procedure to achieve optimal results. In the end, SARA provides an environment for facile extraction of relaxation rates and should promote routine application of accordion relaxation spectroscopy.

PMID:
24408364
PMCID:
PMC4075063
DOI:
10.1007/s10858-013-9807-x
[Indexed for MEDLINE]
Free PMC Article

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