Detection of single-digit foodborne pathogens with the naked eye using carbon nanotube-based multiple cycle signal amplification

Chem Commun (Camb). 2014 Feb 21;50(15):1848-50. doi: 10.1039/c3cc48417c. Epub 2014 Jan 9.

Abstract

A carbon nanotube (CNT)-based multiple cycle signal amplification strategy has been demonstrated for detection of single-digit foodborne pathogens with the naked eye. In the present design, CNTs are used as carriers for loading numerous horseradish peroxidase (HRP) and concanavalin A (ConA) tags, and multiple cycle signal amplification is achieved through the biotinylated anti-HRP antibody and avidin-HRP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Avidin / chemistry
  • Avidin / metabolism
  • Biosensing Techniques / methods*
  • Concanavalin A / chemistry
  • Concanavalin A / metabolism
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Escherichia coli O157 / isolation & purification*
  • Food Microbiology*
  • Horseradish Peroxidase / chemistry
  • Horseradish Peroxidase / metabolism
  • Limit of Detection
  • Meat / microbiology
  • Nanotubes, Carbon / chemistry*
  • Salmonella / isolation & purification*

Substances

  • Nanotubes, Carbon
  • Concanavalin A
  • Avidin
  • Horseradish Peroxidase