Abstract
A carbon nanotube (CNT)-based multiple cycle signal amplification strategy has been demonstrated for detection of single-digit foodborne pathogens with the naked eye. In the present design, CNTs are used as carriers for loading numerous horseradish peroxidase (HRP) and concanavalin A (ConA) tags, and multiple cycle signal amplification is achieved through the biotinylated anti-HRP antibody and avidin-HRP.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Avidin / chemistry
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Avidin / metabolism
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Biosensing Techniques / methods*
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Concanavalin A / chemistry
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Concanavalin A / metabolism
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Enzyme-Linked Immunosorbent Assay / methods*
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Escherichia coli O157 / isolation & purification*
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Food Microbiology*
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Horseradish Peroxidase / chemistry
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Horseradish Peroxidase / metabolism
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Limit of Detection
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Meat / microbiology
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Nanotubes, Carbon / chemistry*
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Salmonella / isolation & purification*
Substances
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Nanotubes, Carbon
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Concanavalin A
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Avidin
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Horseradish Peroxidase