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Insect Mol Biol. 2014 Apr;23(2):244-54. doi: 10.1111/imb.12077. Epub 2014 Jan 9.

Differential gene expression in laboratory strains of human head and body lice when challenged with Bartonella quintana, a pathogenic bacterium.

Author information

1
Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, USA.

Abstract

Human head and body lice are obligatory hematophagous ectoparasites that belong to a single species, Pediculus humanus. Only body lice, however, are vectors of the infectious Gram-negative bacterium Bartonella quintana. Because of their near identical genomes, yet differential vector competence, head and body lice provide a unique model system to study the gain or loss of vector competence. Using our in vitro louse-rearing system, we infected head and body lice with blood containing B. quintana in order to detect both differences in the proliferation of B. quintana and transcriptional differences of immune-related genes in the lice. B. quintana proliferated rapidly in body lice at 6 days post-infection, but plateaued in head lice at 4 days post-infection. RNAseq and quantitative real-time PCR validation analyses determined gene expression differences. Eight immunoresponse genes were observed to be significantly different with many associated with the Toll pathway: Fibrinogen-like protein, Spaetzle, Defensin 1, Serpin, Scavenger receptor A and Apolipoporhrin 2. Our findings support the hypothesis that body lice, unlike head lice, fight infection from B. quintana only at the later stages of its proliferation.

KEYWORDS:

Pediculus humanus capitis; Pediculus humanus humanus; disease vector; ectoparasite; insect; trench fever

PMID:
24404961
PMCID:
PMC4454818
DOI:
10.1111/imb.12077
[Indexed for MEDLINE]
Free PMC Article

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