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Biomicrofluidics. 2013 Sep 17;7(5):54107. doi: 10.1063/1.4821923. eCollection 2013.

Rare cell isolation and profiling on a hybrid magnetic/size-sorting chip.

Author information

1
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, Massachusetts 02114, USA.
2
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, Massachusetts 02114, USA ; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

We present a hybrid magnetic/size-sorting (HMSS) chip for isolation and molecular analyses of circulating tumor cells (CTCs). The chip employs both negative and positive cell selection in order to provide high throughput, unbiased CTC enrichment. Specifically, the system utilizes a self-assembled magnet to generate high magnetic forces and a weir-style structure for cell sorting. The resulting device thus can perform multiple functions, including magnetic depletion, size-selective cell capture, and on-chip molecular staining. With such capacities, the HMSS device allowed one-step CTC isolation and single cell detection from whole blood, tested with spiked cancer cells. The system further facilitated the study of individual CTCs for heterogeneity in molecular marker expression.

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