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Biomicrofluidics. 2013 Aug 5;7(4):44112. doi: 10.1063/1.4817793. eCollection 2013.

Adding reagent to droplets with controlled rupture of encapsulated double emulsions.

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Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California 94158, USA.


We present a method to add reagent to microfluidic droplets by enveloping them as a double emulsions in reagent-filled droplets and then rupturing them with an electric field. When the double emulsions rupture, they release their contents into the enveloping droplets, ensuring mixing with reagent while limiting cross-droplet contamination.

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