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Protein Eng Des Sel. 2014 Feb;27(2):41-7. doi: 10.1093/protein/gzt060. Epub 2014 Jan 8.

A general strategy for antibody library screening via conversion of transient target binding into permanent reporter deposition.

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Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, D-64287 Darmstadt, Germany.


We report here a generally applicable method for the selective covalent attachment of a reporter molecule to a replicating entity that allows one to obtain specific binders from a single round of library screening. We show that selective biotinylation of phage particles displaying a binder to any given target can be achieved by application of a coupled enzyme reaction on the surface of the target-binding phage particles that includes a peroxidase, an oxidase and a catalase. Due to the covalent linkage of biotin together with the tight and stable interaction of biotin with streptavidin, very stringent wash conditions for removal of nonspecific binders can be applied. The method termed (3)CARD (triple catalytic reporter deposition) was successfully applied to single-round screening of a phage display library of camelid single-domain antibodies against three different target proteins.


antibody engineering; catalysed reporter deposition; oxidase; peroxidase; phage display screening

[Indexed for MEDLINE]

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