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Sci Rep. 2014 Jan 8;4:3594. doi: 10.1038/srep03594.

A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.

Author information

1
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
2
Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
3
Institute for Innovation, Ajinomoto CO., Inc., Kawasaki, 210-8681 Japan.
4
1] Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan [2] Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158 USA.

Abstract

In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system.

PMID:
24399248
PMCID:
PMC3884228
DOI:
10.1038/srep03594
[Indexed for MEDLINE]
Free PMC Article

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