Format

Send to

Choose Destination
See comment in PubMed Commons below
Vet Microbiol. 2014 Jan 31;168(2-4):447-50. doi: 10.1016/j.vetmic.2013.11.040. Epub 2013 Dec 14.

Molecular typing of monophasic Salmonella 4,[5]:i:- strains isolated in Belgium (2008-2011).

Author information

1
Veterinary & Agrochemical Research Centre, Foodborne Bacterial Zoonoses & Antibiotic Resistance Unit, Groeselenbergstr. 99, B-1180 Brussels, Belgium.
2
National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health, Wytsmanstreet 14, B-1050 Brussels, Belgium.
3
Scientific Service Food-borne Pathogens, Communicable and Infectious Diseases, Scientific Institute of Public Health, Wytsmanstreet 14, B-1050 Brussels, Belgium.
4
Veterinary & Agrochemical Research Centre, Foodborne Bacterial Zoonoses & Antibiotic Resistance Unit, Groeselenbergstr. 99, B-1180 Brussels, Belgium. Electronic address: piwat@coda-cerva.be.

Abstract

To assess the distribution of Salmonella 4,[5]:i:- subtypes in the Belgian food chain and compare it to the subtypes associated with human infections, a molecular assessment was initiated. Two hundred fifty-three Salmonella isolates serotyped as 4,[5]:i:- during the period 2008-2011 in Belgium and originating from animal productions, food or human clinical samples were analysed by a specific duplex PCR. One hundred ninety-four isolates (76.7%) fit the profile of a S. Typhimurium monophasic variant as defined by the European Food Safety Authority. The other isolates possessed but did not express the phase II flagellin gene (23.3%). Multiple Locus Variable Number of Tandem Repeats Analysis (MLVA) revealed many but closely related profiles in the fljB-negative S. Typhimurium monophasic variant isolates. Some MLVA types were associated with both human and animal isolates but no unique source of human contamination could be demonstrated.

KEYWORDS:

4,[5]:i:-; MLVA; Monophasic; Salmonella; Typhimurium

PMID:
24398228
DOI:
10.1016/j.vetmic.2013.11.040
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center