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EMBO Rep. 2014 Feb;15(2):175-84. doi: 10.1002/embr.201337929. Epub 2014 Jan 7.

Msd1/SSX2IP-dependent microtubule anchorage ensures spindle orientation and primary cilia formation.

Author information

1
Laboratory of Cell Regulation UK, London Research Institute, London, UK.

Abstract

Anchoring microtubules to the centrosome is critical for cell geometry and polarity, yet the molecular mechanism remains unknown. Here we show that the conserved human Msd1/SSX2IP is required for microtubule anchoring. hMsd1/SSX2IP is delivered to the centrosome in a centriolar satellite-dependent manner and binds the microtubule-nucleator γ-tubulin complex. hMsd1/SSX2IP depletion leads to disorganised interphase microtubules and misoriented mitotic spindles with reduced length and intensity. Furthermore, hMsd1/SSX2IP is essential for ciliogenesis, and during zebrafish embryogenesis, knockdown of its orthologue results in ciliary defects and disturbs left-right asymmetry. We propose that the Msd1 family comprises conserved microtubule-anchoring proteins.

PMID:
24397932
PMCID:
PMC3989863
DOI:
10.1002/embr.201337929
[Indexed for MEDLINE]
Free PMC Article

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