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Methods Mol Biol. 2014;1110:323-8. doi: 10.1007/978-1-4614-9408-9_18.

Translating ribosome affinity purification (TRAP) for cell-specific translation profiling in developing flowers.

Author information

1
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Abstract

The development of a multicellular organism is accompanied by cell differentiation. In fact, many biological processes have cell specificity, such that distinct cell types respond differently to endogenous or environmental cues. To obtain cell-specific gene expression profiles, translating ribosome affinity purification (TRAP) has been developed to label polysomes containing translating mRNAs in genetically defined cell types. Here, we describe the immunopurification of epitope-labeled polysomes and associated RNAs from target cell types. TRAP has the additional advantage of obtaining only translating mRNAs, which are a better proxy to the proteome than a standard mRNA preparation.

PMID:
24395267
DOI:
10.1007/978-1-4614-9408-9_18
[Indexed for MEDLINE]

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