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Muscle Nerve. 2014 Jun;49(6):919-21. doi: 10.1002/mus.24160.

Effect of recombinant human MG53 protein on tourniquet-induced ischemia-reperfusion injury in rat muscle.

Author information

1
United States Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, 3698 Chambers Pass, Fort Sam Houston, Texas, 78234.

Abstract

INTRODUCTION:

Skeletal muscle ischemia-reperfusion injury (I-R) is a complex injury process that includes damage to the sarcolemmal membrane, contributing to necrosis and apoptosis. MG53, a muscle-specific TRIM family protein, has been shown to be essential for regulating membrane repair and has been shown to be protective against cardiac I-R and various forms of skeletal muscle injury. The purpose of this study was to determine if recombinant human MG53 (rhMG53) administration offered protection against I-R.

METHODS:

rhMG53 was administered to rats immediately before tourniquet-induced ischemia and again immediately before reperfusion. Two days later muscle damage was assessed histologically.

RESULTS:

rhMG53 offered no protective effect, as evidenced primarily by similar Evans blue dye inclusion in the muscles of rats administered rhMG53 or saline.

CONCLUSIONS:

Administration of rhMG53 does not offer protection against I-R in rat skeletal muscle. Additional studies are required to determine if the lack of a response is species-specific.

KEYWORDS:

MG53; TRIM72; ischemia-reperfusion; muscle injury; tourniquet

PMID:
24395153
PMCID:
PMC4028410
DOI:
10.1002/mus.24160
[Indexed for MEDLINE]
Free PMC Article

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