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Mod Pathol. 2014 Aug;27(8):1101-15. doi: 10.1038/modpathol.2013.240. Epub 2014 Jan 3.

Improved detection reveals active β-papillomavirus infection in skin lesions from kidney transplant recipients.

Author information

1
Virology Unit, Department of Translational Medicine, Medical School of Novara, Novara, Italy.
2
1] Virology Unit, Department of Translational Medicine, Medical School of Novara, Novara, Italy [2] Department of Public Health and Pediatric Sciences, Medical School of Turin, Turin, Italy.
3
Dermatology Unit, Department of Translational Medicine, Medical School of Novara, Novara, Italy.
4
Nephrology Unit, Department of Translational Medicine, Medical School of Novara, Novara, Italy.
5
Pathology Unit, Department of Health Sciences, Medical School of Novara, Novara, Italy.
6
Division of Virology, National Institute for Medical Research, London, UK.
7
Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands.
8
1] Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands [2] DDL Diagnostic Laboratory, Rijswijk, The Netherlands.
9
DDL Diagnostic Laboratory, Rijswijk, The Netherlands.
10
Department of Public Health and Pediatric Sciences, Medical School of Turin, Turin, Italy.

Erratum in

  • Mod Pathol. 2014 Jun;27(6):917.

Abstract

The aim of this study was to determine whether detection of β-HPV gene products, as defined in epidermodysplasia verruciformis skin cancer, could also be observed in lesions from kidney transplant recipients alongside the viral DNA. A total of 111 samples, corresponding to 79 skin lesions abscised from 17 kidney transplant recipients, have been analyzed. The initial PCR analysis demonstrated that β-HPV-DNA was highly present in our tumor series (85%). Using a combination of antibodies raised against the E4 and L1 proteins of the β-genotypes, we were able to visualize productive infection in 4 out of 19 actinic keratoses, and in the pathological borders of 1 out of 14 squamous cell carcinomas and 1 out of 31 basal cell carcinomas. Increased expression of the cellular proliferation marker minichromosome maintenance protein 7 (MCM7), that extended into the upper epithelial layers, was a common feature of all the E4-positive areas, indicating that cells were driven into the cell cycle in areas of productive viral infections. Although the present study does not directly demonstrate a causal role of these viruses, the detection of E4 and L1 positivity in actinic keratosis and the adjacent pathological epithelium of skin cancer, clearly shows that β-HPV are actively replicating in the intraepidermal precursor lesions of kidney transplant recipients and can therefore cooperate with other carcinogenic agents, such as UVB, favoring skin cancer promotion.

PMID:
24390217
DOI:
10.1038/modpathol.2013.240
[Indexed for MEDLINE]
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