Format

Send to

Choose Destination
See comment in PubMed Commons below
Behav Brain Res. 2014 Mar 15;261:210-9. doi: 10.1016/j.bbr.2013.12.032. Epub 2013 Dec 31.

Voluntary forced use of the impaired limb following stroke facilitates functional recovery in the rat.

Author information

1
Departments of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI, C1A4P3, Canada.
2
Departments of Biology, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI, C1A4P3, Canada.
3
Departments of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI, C1A4P3, Canada. Electronic address: tasker@upei.ca.

Abstract

Constraint induced movement therapy (CIMT), which forces use of the impaired arm following stroke, improves functional recovery. The mechanisms underlying recovery are not well understood, necessitating further investigation into how rehabilitation may affect neuroplasticity using animal models. Animal motivation and stress make modelling CIMT in animals challenging. We have shown that following focal ischemia, voluntary forced use therapy using pet activity balls could engage the impaired forelimb and result in a modest acceleration in recovery. In this study, we investigated the effects of a more intensive appetitively motivated regimen that included task specific reaching exercises. Adult male Sprague Dawley rats were subjected to focal unilateral stroke using intracerebral injections of endothelin-1 or sham surgery. Three days later, stroke animals were assigned to daily rehabilitation or control therapy. Rehabilitation consisted of 30 min of generalized movement sessions in activity balls, followed by 30 min of voluntary task-specific movement using reaching boxes. Rats were tested weekly to measure forelimb deficit and recovery. After 30 days, animals were euthanized and tissue was examined for infarct volume, brain derived neurotrophic factor expression, and the presence of new neurons using doublecortin immunohistochemistry. Rehabilitation resulted in a significant acceleration of forelimb recovery in several tests, and a significant increase in the number of doublecortin-expressing cells. Furthermore, while the proportion of cells expressing BDNF in the peri-infarct region did not change, there was a shift in the cellular origin of expressed BDNF, resulting in significantly more non-neuronal, non-astrocytic BDNF, presumed to be of microglial origin.

KEYWORDS:

Constraint induced movement therapy; Endothelin-1; Forced use; Neuroplasticity; Rehabilitation; Stroke

PMID:
24388978
DOI:
10.1016/j.bbr.2013.12.032
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center