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Plant Sci. 2014 Feb;215-216:117-23. doi: 10.1016/j.plantsci.2013.11.007. Epub 2013 Nov 16.

TamiR1123 originated from a family of miniature inverted-repeat transposable elements (MITE) including one inserted in the Vrn-A1a promoter in wheat.

Author information

1
Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
2
Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078, USA. Electronic address: liuling.yan@okstate.edu.

Abstract

More than half of spring wheat cultivars have a dominant Vrn-A1a allele that has an insertion of a miniature inverted-repeat transposable element (MITE) in its promoter. In this study, we found that the MITE present in the Vrn-A1a gene (MITE_VRN) is a nearly perfect palindrome and it can form highly stable hairpin loops when expressed as RNA. MITE_VRN also possessed sequences of a microRNA in Triticum aestivum (TamiR1123). The P(32) labeled TamiR1123 probe detected two RNA molecules on a small RNA gel blot, one expected for MITE_VRN, and the other expected for TamiR1123. These results demonstrated that MITE_VRN was expressed as RNAs and TamiR1123 was originated from the MITE_VRN family. The isogenic line TDD carrying the dominant Vrn-A1a allele with MITE_VRN showed higher TamiR1123 and Vrn-A1a transcript levels than the isogenic line TDE carrying the recessive vrn-A1a allele without MITE_VRN. TamiR1123 were greatly up-regulated by plant age but slightly down-regulated by low temperature and short days. These findings have pointed to alternative regulatory mechanisms for plant development governed by Vrn-A1a in spring wheat.

KEYWORDS:

Flowering time; MITE; VRN1; Vernalization; Wheat; microRNA

PMID:
24388522
DOI:
10.1016/j.plantsci.2013.11.007
[Indexed for MEDLINE]
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