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Cell Stem Cell. 2014 Jan 2;14(1):13-26. doi: 10.1016/j.stem.2013.12.005.

Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics.

Author information

1
NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: cheng@mail.nih.gov.
2
NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
3
The Lieber Institute for Brain Development, 855 North Wolfe Street, Baltimore, MD 21205, USA.
4
Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, 3D environmental cues, and modes of multicellular association. In this Protocol Review, we dissect these growth components by comparing cell culture methods and identifying the benefits and pitfalls associated with each one. We further provide criteria, considerations, and suggestions to achieve optimal cell growth for hPSC expansion, differentiation, and use in future therapeutic applications.

PMID:
24388173
PMCID:
PMC3915741
DOI:
10.1016/j.stem.2013.12.005
[Indexed for MEDLINE]
Free PMC Article

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