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PLoS One. 2013 Dec 26;8(12):e83409. doi: 10.1371/journal.pone.0083409. eCollection 2013.

Determination of HER2 amplification status on tumour DNA by digital PCR.

Author information

1
The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, London, United Kingdom.
2
The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, London, United Kingdom ; Breast Unit, Royal Marsden Hospital, London, United Kingdom.

Abstract

Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2:EFTDU2 ratio by digital PCR assay in the microdissected DNA from 18 HER2 amplified and 58 HER2 non-amplified cancers. The HER2:EFTUD2 ratio had high concordance with conventionally defined HER2 status with a sensitivity of 100% (18/18) and a specificity of 98% (57/58). The HER2:EFTUD2 digital PCR assay has potential to accurately assess HER2 amplification status.

PMID:
24386193
PMCID:
PMC3873285
DOI:
10.1371/journal.pone.0083409
[Indexed for MEDLINE]
Free PMC Article

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