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Bioresour Technol. 2014 Feb;153:388-92. doi: 10.1016/j.biortech.2013.12.030. Epub 2013 Dec 14.

Two amplicon sequencing strategies revealed different facets of the prokaryotic community associated with the anaerobic treatment of vinasses from ethanol distilleries.

Author information

1
PROIMI-CONICET, Av. Belgrano y Pje. Caseros, SM de Tucumán 4000, Tucumán, Argentina; Facultad de Ciencias Exactas y Tecnología, Universidad Nacional de Tucumán, Av. Independencia 1800, SM de Tucumán 4000, Tucumán, Argentina. Electronic address: ale.martinez.unt@gmail.com.
2
Laboratorio de Organización y Evolución del Genoma, Dpto. Ecología y Evolución, Facultad de Ciencias/CURE, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay. Electronic address: eletor@fcien.edu.uy.
3
PROIMI-CONICET, Av. Belgrano y Pje. Caseros, SM de Tucumán 4000, Tucumán, Argentina; Facultad de Ciencias Exactas y Tecnología, Universidad Nacional de Tucumán, Av. Independencia 1800, SM de Tucumán 4000, Tucumán, Argentina. Electronic address: nperotti@herrera.unt.edu.ar.

Abstract

The prokaryotic consortium from a pilot-scale UASB reactor fed with vinasses from ethanol distilleries was evaluated by means of amplicon sequencing of the 16S rRNA gene. Two different sets of primers targeted to overlapping regions of the V4-16S region were used to gain a broad picture of such community and to perform a comparative analysis. From the two datasets obtained, prevalent phyla were Firmicutes, Verrucomicrobia and Thermotogae. Interestingly, one set of primers captured variability in both the bacterial and archaeal portions of the community, whilst the other one revealed a more diverse community structure, but only in the Bacteria domain. Although a certain level of agreement between the two strategies was observed, sharp differences indicate that different facets of the community were disclosed by each approach.

KEYWORDS:

16S rRNA pyrosequencing; Anaerobic digestion; Community profiling; Vinasses

PMID:
24382487
DOI:
10.1016/j.biortech.2013.12.030
[Indexed for MEDLINE]

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