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Elife. 2013 Dec 31;2:e01256. doi: 10.7554/eLife.01256.

Chromatin signature of widespread monoallelic expression.

Author information

1
Department of Cancer Biology and Center for Cancer Systems Biology, Dana-Farber Cancer Institute, Boston, United States.

Abstract

In mammals, numerous autosomal genes are subject to mitotically stable monoallelic expression (MAE), including genes that play critical roles in a variety of human diseases. Due to challenges posed by the clonal nature of MAE, very little is known about its regulation; in particular, no molecular features have been specifically linked to MAE. In this study, we report an approach that distinguishes MAE genes in human cells with great accuracy: a chromatin signature consisting of chromatin marks associated with active transcription (H3K36me3) and silencing (H3K27me3) simultaneously occurring in the gene body. The MAE signature is present in ∼20% of ubiquitously expressed genes and over 30% of tissue-specific genes across cell types. Notably, it is enriched among key developmental genes that have bivalent chromatin structure in pluripotent cells. Our results open a new approach to the study of MAE that is independent of polymorphisms, and suggest that MAE is linked to cell differentiation. DOI: http://dx.doi.org/10.7554/eLife.01256.001.

KEYWORDS:

AST-Seq; bivalent; chromatin; epigenome; monoallelic expression

PMID:
24381246
PMCID:
PMC3873816
DOI:
10.7554/eLife.01256
[Indexed for MEDLINE]
Free PMC Article

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